protein chemistry

Selective protein chemistry makes it possible to attach useful probes or isolate a protein while preserving its biological role. Research on the N-terminal glycine residue established a route to single-site protein labelling. Building on this chemistry, the Gly-tag approach enables selective capture and release for protein purification without a metal affinity tag.

1. Purushottam, L.+; Unnikrishnan, V. B.+; Gujrati, M.; Adusumalli, S.; Rawale, D. G.; Mishra, R. K.; Rai, V. Single amino acid Gly-tag enables metal-free protein purification. Chem. Sci. 2020, 11, 13137-13142.
   (+Equal first-author contribution)
   (Part of themed collection: Most Popular 2019-2020 Chemical Biology Articles of Chem. Sci)
   ( Gly-Tag® purification resin @Plabeltech)
   
   
2. Purushottam, L.; Adusumalli, S.; Singh, U.; Unnikrishnan, V. B.; Rawale, D. G.; Gujrati, M.; Mishra, R. K.; Rai, V. Single-site glycine-specific labeling of proteins. Nat. Commun. 2019, 10, 1100-1103.
   (Featured in Editors’ Highlights of Nature Communications)